PT  - JOURNAL ARTICLE
AU  - Simon van Vliet
AU  - Alma Dal Co
AU  - Annina R. Winkler
AU  - Stefanie Spriewald
AU  - Bärbel Stecher
AU  - Martin Ackermann
TI  - Local interactions lead to spatially correlated gene expression levels in bacterial groups
AID  - 10.1101/109991
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 109991
4099  - http://biorxiv.org/content/early/2017/02/20/109991.short
4100  - http://biorxiv.org/content/early/2017/02/20/109991.full
AB  - Many bacteria live in spatially structured assemblies where the microenvironment of a cell is shaped by the activities of its neighbors. Bacteria regulate their gene expression based on the inferred state of the environment. This raises the question whether the phenotypes of neighboring cells can become correlated through interactions via the shared microenvironment. Here, we addressed this question by following gene expression dynamics in Escherichia coli microcolonies. We observed strong spatial correlations in the expression dynamics for pathways involved in toxin production, SOS-stress response, and metabolism. These correlations can partly be explained by a combination of shared lineage history and spatial gradients in the colony. Interestingly, we also found evidence for cell-cell interactions in SOS-stress response, methionine biosynthesis and overall metabolic activity. Together our data suggests that intercellular feedbacks can couple the phenotypes of neighboring cells, raising the question whether gene-regulatory networks have evolved to spatially organize biological functions.