RT Journal Article
SR Electronic
T1 SAVE: A secure cloud-based pipeline for CRISPR pooled screen deconvolution
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 110262
DO 10.1101/110262
A1 Hyun-Hwan Jeong
A1 Seon Young Kim
A1 Maxime WC Rosseaux
A1 Huda Y Zoghbi
A1 Zhandong Liu
YR 2017
UL http://biorxiv.org/content/early/2017/02/21/110262.abstract
AB We present a user-friendly, cloud-based, data analysis pipeline for the deconvolution of pooled screening data. This tool, termed SAVE for Screening Analysis Visual Explorer, serves a dual purpose of extracting, clustering and analyzing raw next generation sequencing files derived from pooled screening experiments while at the same time presenting them in a user-friendly way on a secure web-based platform. Moreover, SAVE serves as a useful web-based analysis pipeline for reanalysis of pooled CRISPR screening datasets. Taken together, the framework described in this study is expected to accelerate development of web-based bioinformatics tool for handling all studies which include next generation sequencing data. SAVE is available at http://save.nrihub.org.