RT Journal Article
SR Electronic
T1 Large-scale simultaneous measurement of epitopes and transcriptomes in single cells
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 113068
DO 10.1101/113068
A1 Marlon Stoeckius
A1 Christoph Hafemeister
A1 William Stephenson
A1 Brian Houck-Loomis
A1 Harold Swerdlow
A1 Rahul Satija
A1 Peter Smibert
YR 2017
UL http://biorxiv.org/content/early/2017/03/02/113068.abstract
AB Recent high-throughput single-cell sequencing approaches have been transformative for understanding complex cell populations, but are unable to provide additional phenotypic information, such as protein levels of well-established cell-surface markers. Using oligonucleotide-labeled antibodies, we integrate measurements of cellular proteins and transcriptomes into an efficient, sequencing-based readout of single cells. This method is compatible with existing single-cell sequencing approaches and will readily scale as the throughput of these methods increase.