RT Journal Article
SR Electronic
T1 MicroRNA exocytosis by large dense-core vesicle fusion
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 093278
DO 10.1101/093278
A1 Alican Gümürdü
A1 Ramazan Yildiz
A1 Erden Eren
A1 Gökhan Karakülah
A1 Turgay Ünver
A1 Şermin Genç
A1 Yongsoo Park
YR 2017
UL http://biorxiv.org/content/early/2017/03/08/093278.abstract
AB Neurotransmitters and peptide hormones are secreted into outside the cell by a vesicle fusion process. Although non-coding RNA (ncRNA) that include microRNA (miRNA) regulates gene expression inside the cell where they are transcribed, extracellular miRNA has been recently discovered outside the cells, proposing that miRNA might be released to participate in cell-to-cell communication. Despite its importance of extracellular miRNA, the molecular mechanisms by which miRNA can be stored in vesicles and released by vesicle fusion remain enigmatic. Using next-generation sequencing, vesicle purification techniques, and synthetic neurotransmission, we observe that large dense-core vesicles (LDCVs) contain a variety of miRNAs including miR-375. Furthermore, miRNA exocytosis is mediated by the SNARE complex and accelerated by Ca2+. Our results suggest that miRNA can be a novel neuromodulator that can be stored in vesicles and released by vesicle fusion together with classical neurotransmitters.One Sentence Summary Using next-generation sequencing (NGS) for microRNA (miRNA) and synthetic neurotransmission, we observed that large dense-core vesicles (LDCVs) contain a variety of miRNA together with classical neurotransmitters, and that miRNA can be released by vesicle fusion mediated by SNARE.