RT Journal Article
SR Electronic
T1 A modified GC-specific MAKER gene annotation method reveals improved and novel gene predictions of high and low GC content in <em>Oryza sativa</em>
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 115345
DO 10.1101/115345
A1 Megan J. Bowman
A1 Jane A. Pulman
A1 Tiffany L. Liu
A1 Kevin L. Childs
YR 2017
UL http://biorxiv.org/content/early/2017/03/09/115345.abstract
AB Accurate structural annotation depends on well-trained gene prediction programs. Training data for gene prediction programs are often chosen randomly from a subset of high-quality genes that ideally represent the variation found within a genome. One aspect of gene variation is GC content, which differs across species and is bimodal in grass genomes. We find that gene prediction programs trained on genes with random GC content do not completely predict all grass genes with extreme GC content. We present a new GC-specific MAKER annotation protocol to predict new and improved gene models and assess the biological significance of this method in Oryza sativa.