TY - JOUR T1 - First landscape of binding to chromosomes for a domesticated mariner transposase in the human genome: diversity of genomic targets of SETMAR isoforms in two colorectal cell lines JF - bioRxiv DO - 10.1101/115030 SP - 115030 AU - Aymeric Antoine-Lorquin AU - Ahmed Arnaoty AU - Sassan Asgari AU - Martine Batailler AU - Linda Beauclair AU - Catherine Belleannée AU - Solenne Bire AU - Nicolas Buisine AU - Vincent Coustham AU - Alban Girault AU - Serge Guyetant AU - Thierry Lecomte AU - Benoît Piégu AU - Bruno Pitard AU - Isabelle Stévant AU - Yves Bigot Y1 - 2017/01/01 UR - http://biorxiv.org/content/early/2017/03/09/115030.abstract N2 - Setmar is a 3-exons gene coding a SET domain fused to a Hsmar1 transposase. Its different transcripts theoretically encode 8 isoforms with SET moieties differently spliced. In vitro, the largest isoform binds specifically to Hsmar1 DNA ends and with no specificity to DNA when it is associated with hPso4. In colon cell lines, we found they bind specifically to two chromosomal targets depending probably on the isoform, Hsmar1 ends and sites with no conserved motifs. We also discovered that the isoforms profile was different between cell lines and patient tissues, suggesting the isoforms encoded by this gene in healthy cells and their functions are currently not investigated. ER -