TY - JOUR T1 - Rapid genome recoding by iterative recombineering of synthetic DNA JF - bioRxiv DO - 10.1101/115493 SP - 115493 AU - Yu Heng Lau AU - Finn Stirling AU - James Kuo AU - Michiel A.P. Karrenbelt AU - Yujia A. Chan AU - Adam Riesselman AU - Connor A. Horton AU - Elena Schaefer AU - David Lips AU - Matthew T. Weinstock AU - Daniel G. Gibson AU - Jeffrey C. Way AU - Pamela A. Silver Y1 - 2017/01/01 UR - http://biorxiv.org/content/early/2017/03/09/115493.abstract N2 - Genome recoding will provide a deeper understanding of genetics and transform biotechnology. We bypass the reliance of previous genome recoding methods on site-specific enzymes and demonstrate a rapid recombineering based strategy for writing genomes by Stepwise Integration of Rolling Circle Amplified Segments (SIRCAS). We installed the largest number of codon substitutions in a single organism yet published, creating a strain of Salmonella typhimurium with 1557 leucine codon changes across 200 kb of the genome. ER -