TY - JOUR T1 - Compositionally distinct nuclear pore complexes of functionally distinct dimorphic nuclei in ciliate <em>Tetrahymena</em> JF - bioRxiv DO - 10.1101/116277 SP - 116277 AU - Masaaki Iwamoto AU - Hiroko Osakada AU - Chie Mori AU - Yasuhiro Fukuda AU - Koji Nagao AU - Chikashi Obuse AU - Yasushi Hiraoka AU - Tokuko Haraguchi Y1 - 2017/01/01 UR - http://biorxiv.org/content/early/2017/03/13/116277.abstract N2 - SUMMARY STATEMENT Our study demonstrates compositional and structural differences of the nuclear pore complex between the functionally differentiated macronucleus and micronucleus within a single cytoplasm of ciliated protozoa.ABSTRACT The nuclear pore complex (NPC), a gateway for nucleocytoplasmic trafficking, is composed of about 30 different proteins called nucleoporins. It remains unknown whether the NPCs within a species are homogeneous or vary depending on the cell type, or physiological condition. Here, we present evidence for compositionally distinct NPCs that form within a single cell in a binucleated ciliate. In Tetrahymena thermophila, each cell contains both a transcriptionally-active macronucleus (MAC) and a germline micronucleus (MIC). By combining in silico analysis, mass spectrometry analysis for immuno-isolated proteins, and subcellular localization analysis of GFP fused proteins, we identified numerous novel components of MAC and MIC NPCs. Core members of the Nup107-160 scaffold complex were enriched in MIC NPCs. Strikingly, two paralogs of Nup214 and of Nup153 localized exclusively to either MAC or MIC NPCs. Furthermore, the transmembrane components Pom121 and Pom82 localize exclusively to MAC and MIC NPCs, respectively. Our results argue that functional nuclear dimorphism in ciliates is likely to depend on compositional and structural specificity of NPCs. ER -