PT  - JOURNAL ARTICLE
AU  - Niels R. Ntamati
AU  - Meaghan Creed
AU  - Christian Lüscher
TI  - Periaqueductal efferents to dopamine and GABA neurons of the VTA
AID  - 10.1101/117416
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 117416
4099  - http://biorxiv.org/content/early/2017/03/16/117416.short
4100  - http://biorxiv.org/content/early/2017/03/16/117416.full
AB  - Neurons in the periaqueductal gray (PAG) modulate threat responses and nociception. Activity in the ventral tegmental area (VTA) on the other hand can cause reinforcement and aversion. While in many situations these behaviors are related, the anatomical substrate of a crosstalk between the PAG and VTA remains poorly understood. Here we describe the anatomical and electrophysiological organization of the VTA-projecting PAG neurons. Using rabies-based, cell type-specific retrograde tracing, we observed that PAG to VTA projection neurons are evenly distributed along the rostro-caudal axis of the PAG, but concentrated in its posterior and ventrolateral segments. Optogenetic projection targeting demonstrated that the PAG-to-VTA pathway is predominantly excitatory and targets similar proportions of Ih-expressing VTA DA and GABA neurons. Taken together, these results set the framework for functional analysis of the interplay between PAG and VTA in the regulation of reward and aversion.