RT Journal Article
SR Electronic
T1 Genome-wide Segregation of Single Nucleotide and Structural Variants into Single Cancer Cells
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 117663
DO 10.1101/117663
A1 John Easton
A1 Veronica Gonzalez Pena
A1 Donald Yergeau
A1 Xiaotu Ma
A1 Charles Gawad
YR 2017
UL http://biorxiv.org/content/early/2017/03/16/117663.abstract
AB We present a new approach for determining comprehensive variant profiles of single cells using a microfluidic amplicon-based strategy. This method can be used to reconstruct the clonal architecture and mutational history of a malignancy using all classes and sizes of single nucleotide and structural variants, providing insights into the temporal changes in mutational classes and processes that led to the development of a cancer. Using this approach, we interrogated single cells from a patient with leukemia, determining that processes producing structural variation preceded single nucleotides changes in the development of that malignancy.