RT Journal Article
SR Electronic
T1 BEARscc: robustness of single-cell clusters determined using simulated technical replicates
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 118919
DO 10.1101/118919
A1 DT Severson
A1 RP Owen
A1 MJ White
A1 X Lu
A1 B Schuster-Böckler
YR 2017
UL http://biorxiv.org/content/early/2017/03/21/118919.abstract
AB Technical variance is a major confounding factor in single-cell RNA sequencing, not least because measurements on the same cell are not replicable. We developed BEARscc, a tool that simulates experiment-specific technical replicates based on a probabilistic model of technical variance trained on RNA spike-in measurements. We demonstrate that the tool improves the unsupervised classification of cells and aids the interpretation of single-cell RNA-seq experiments.