TY - JOUR T1 - UBiT2: a client-side web-application for gene expression data analysis JF - bioRxiv DO - 10.1101/118992 SP - 118992 AU - Jean Fan AU - David Fan AU - Kamil Slowikowski AU - Nils Gehlenborg AU - Peter Kharchenko Y1 - 2017/01/01 UR - http://biorxiv.org/content/early/2017/03/22/118992.abstract N2 - We present a purely client-side web-application, UBiT2 (User-friendly BioInformatics Tools), that provides installation-free, offline alignment, analysis, and visualization of RNA-sequencing as well as qPCR data. Analysis modules were designed with single cell transcriptomic analysis in mind. Using just a browser, users can perform standard analyses such as quality control, filtering, hierarchical clustering, principal component analysis, differential expression analysis, gene set enrichment testing, and more, all with interactive visualizations and exportable publication-quality figures. We apply UBiT2 to recapitulate findings from single cell RNA-seq and Fluidigm Biomarkā„¢ multiplex RT-qPCR gene expression datasets. UBiT2 is available at http://pklab.med.harvard.edu/jean/ubit2/index.html with open-source code available at https://github.com/JEFworks/ubit2. ER -