RT Journal Article
SR Electronic
T1 Molecular Counting with Localization Microscopy: A Bayesian estimate based on single fluorophore statistics
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 071191
DO 10.1101/071191
A1 D. Nino
A1 N. Rafiei
A1 Y. Wang
A1 A. Zilman
A1 J. N. Milstein
YR 2017
UL http://biorxiv.org/content/early/2017/03/22/071191.abstract
AB Super-resolved localization microscopy (SLM) has the potential to serve as an accurate, singlecell technique for counting the abundance of intracellular molecules. However, the stochastic blinking of single fluorophores can introduce large uncertainties into the final count. Here we provide a theoretical foundation for applying SLM to the problem of molecular counting based on the distribution of blinking events from a single fluorophore. We also show that by redundantly tagging single-molecules with multiple, blinking fluorophores, the accuracy of the technique can be enhanced by harnessing the central limit theorem. The coefficient of variation (CV) then, for the number of molecules M estimated from a given number of blinks B, scales like , where Nl is the mean number of labels on a target. As an example, we apply our theory to the challenging problem of quantifying the cell-to-cell variability of plasmid copy number in bacteria.