PT  - JOURNAL ARTICLE
AU  - Vicki A. M. Gold
AU  - Piotr Chroscicki
AU  - Piotr Bragoszewski
AU  - Agnieszka Chacinska
TI  - Cytosolic ribosomes on the surface of mitochondria
AID  - 10.1101/119339
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 119339
4099  - http://biorxiv.org/content/early/2017/03/22/119339.short
4100  - http://biorxiv.org/content/early/2017/03/22/119339.full
AB  - By electron cryo-tomography and subtomogram averaging, translation-arrested ribosomes were used to depict the clustered organisation of the TOM complex on the surface of mitochondria, corroborating earlier reports of localized translation. Ribosomes were shown to interact specifically with the TOM complex and nascent chain binding was crucial for ribosome recruitment and stabilization. Ribosomes were bound to the membrane in discrete clusters, often in the vicinity of the crista junctions. This interaction highlights how protein synthesis may be coupled with transport, and the importance of spatial organization for efficient mitochondrial protein import.