PT  - JOURNAL ARTICLE
AU  - Magalie Martineau
AU  - Agila Somasundaram
AU  - Jonathan B. Grimm
AU  - Todd D. Gruber
AU  - Daniel Choquet
AU  - Justin W. Taraska
AU  - Luke D. Lavis
AU  - David Perrais
TI  - Semisynthetic pH-sensitive fluorophores for imaging exocytosis and endocytosis
AID  - 10.1101/118505
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 118505
4099  - http://biorxiv.org/content/early/2017/03/25/118505.short
4100  - http://biorxiv.org/content/early/2017/03/25/118505.full
AB  - The GFP-based superecliptic pHluorin (SEP) enables detection of exocytosis and endocytosis, but its performance has not been duplicated in red fluorescent protein scaffolds. Here we describe ‘semisynthetic’ pH-sensitive protein conjugates that match the properties of SEP. Conjugation to genetically encoded self-labeling tags or antibodies allows visualization of both exocytosis and endocytosis, constituting new bright sensors for these key steps of synaptic transmission.