PT  - JOURNAL ARTICLE
AU  - Anders Eriksson
AU  - Marlena Raczkowska
AU  - Rapeechai Navawongse
AU  - Deepak Choudhury
AU  - James C. Stewart
AU  - Yi Ling Tang
AU  - Zhiping Wang
AU  - Adam Claridge-Chang
TI  - Neuromodulatory circuit effects on <em>Drosophila</em> feeding behaviour and metabolism
AID  - 10.1101/086413
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 086413
4099  - http://biorxiv.org/content/early/2017/04/09/086413.short
4100  - http://biorxiv.org/content/early/2017/04/09/086413.full
AB  - Animals have evolved to maintain homeostasis in a changing external environment by adapting their internal metabolism and behaviour. Metabolism and feeding behaviour are coordinated by neuromodulation; a number of the implicated neuromodulatory systems are homologous between mammals and an important neurogenetic model, the vinegar fly. We investigated whether silencing neuromodulatory networks would elicit coordinated changes in feeding, behavioural activity and metabolism. We employed transgenic fly lines that allowed us to inhibit broad cellular sets of the dopaminergic, serotonergic, octopaminergic, tyraminergic and neuropeptide F systems. The genetically-manipulated animals were then assessed for changes in their overt behavioural responses and metabolism by monitoring eleven parameters: activity; climbing ability; individual feeding; group feeding; food discovery; both fed and starved respiration; fed and starved lipid content;and fed/starved body weight. The results from these 55 experiments indicate that individual neuromodulatory system effects on feeding behaviour, motor activity and metabolism are dissociated.