TY - JOUR T1 - Challenges and emerging directions in single-cell analysis JF - bioRxiv DO - 10.1101/127761 SP - 127761 AU - Guo-Cheng Yuan AU - Long Cai AU - Michael Elowitz AU - Tariq Enver AU - Guoping Fan AU - Guoji Guo AU - Rafael Irizarry AU - Peter Kharchenko AU - Junhyong Kim AU - Stuart Orkin AU - John Quackenbush AU - Assieh Saadatpour AU - Timm Schroeder AU - Ramesh Shivdasani AU - Itay Tirosh Y1 - 2017/01/01 UR - http://biorxiv.org/content/early/2017/04/15/127761.abstract N2 - Single-cell analysis is a rapidly evolving approach to characterize genome-scale molecular information at the individual cell level. Development of single-cell technologies and computational methods has enabled systematic investigation of cellular heterogeneity in a wide range of tissues and cell populations, yielding fresh insights into the composition, dynamics, and regulatory mechanisms of cell states in development and disease. Despite substantial advances, significant challenges remain in the analysis, integration, and interpretation of single-cell omics data. Here, we discuss the state of the field and recent advances, and look to future opportunities. ER -