TY - JOUR T1 - STRT-seq-2i: dual-index 5′ single cell and nucleus RNA-seq on an addressable microwell array JF - bioRxiv DO - 10.1101/126268 SP - 126268 AU - Hannah Hochgerner AU - Peter Lönnerberg AU - Rebecca Hodge AU - Jaromir Mikes AU - Abeer Heskol AU - Hermann Hubschle AU - Philip Lin AU - Simone Picelli AU - Gioele La Manno AU - Michael Ratz AU - Jude Dunne AU - Syed Husain AU - Ed Lein AU - Maithreyan Srinivasan AU - Amit Zeisel AU - Sten Linnarsson Y1 - 2017/01/01 UR - http://biorxiv.org/content/early/2017/04/20/126268.abstract N2 - Single-cell RNA-seq has become routine for discovering cell types and revealing cellular diversity, but currently no high-throughput platform has been used successfully on archived human brain samples. We present STRT-seq-2i, an addressable 9600-microwell array platform, combining sampling by limiting dilution or FACS, with imaging and high throughput at competitive cost. We applied the platform to fresh single mouse cortical cells and to frozen post-mortem human cortical nuclei, matching the performance of a previous lower-throughput platform. ER -