TY - JOUR T1 - A practical guide to CRISPR/Cas9 genome editing in Lepidoptera JF - bioRxiv DO - 10.1101/130344 SP - 130344 AU - Linlin Zhang AU - Robert D. Reed Y1 - 2017/01/01 UR - http://biorxiv.org/content/early/2017/04/24/130344.abstract N2 - CRISPR/Cas9 genome editing has revolutionized functional genetic work in many organisms and is having an especially strong impact in emerging model systems. Here we summarize recent advances applying CRISPR/Cas9 methods in Lepidoptera, with a focus on providing practical advice on the entire process of genome editing from experimental design through to genotyping. We also describe successful targeted GFP knock-ins that we have achieved in butterflies. Finally, we provide a complete, detailed protocol for producing targeted long deletions in butterflies. ER -