%0 Journal Article %A Henry H. Lee %A Nili Ostrov %A Michaela A. Gold %A George M. Church %T Recombineering in Vibrio natriegens %D 2017 %R 10.1101/130088 %J bioRxiv %P 130088 %X Here, we show that λ-Red homologs found in the Vibrio-associated SXT mobile element potentiate allelic exchange in V. natriegens by ~10,000-fold. Specifically, we show SXT-Beta (s065), SXT-Exo (s066), and λ-Gam proteins are sufficient to enable recombination of single- and double-stranded DNA with episomal and genomic loci. We characterize and optimize episomal oligonucleotide-mediated recombineering and demonstrate recombineering at genomic loci. We further show targeted genomic deletion of the extracellular nuclease gene dns using a double-stranded DNA cassette. Continued development of this recombination technology will advance high-throughput and large-scale genetic engineering efforts to domesticate V. natriegens and to investigate its rapid growth rate. %U https://www.biorxiv.org/content/biorxiv/early/2017/04/26/130088.full.pdf