@article {Teng131102, author = {Shuaishuai Teng and Yang Li and Ming Yang and Rui Qi and Yiming Huang and Qianyu Wang and Shasha Li and Kequan Lin and Yujing Cheng and Zhi John Lu and Dong Wang}, title = {Epigenetic Reprogramming of Tissue-Specific Transcription Promotes Metastasis}, elocation-id = {131102}, year = {2017}, doi = {10.1101/131102}, publisher = {Cold Spring Harbor Laboratory}, abstract = {Tumor metastasis is the cause of death for 90\% of cancer patients, and no currently-available therapies target this multi-step process in which cancer cells spread from the local tissue of a primary tumor to distant organs where they establish secondary tumors1. Although epithelial-to-mesenchymal transition2, tumor-secreted exosomes3, epigenetic regulators as well as other genes4-8 have been implicated in metastasis, little is known about how cells adapt to and colonize new tissue environments. Here, we show that the epigenetics-mediated reprogramming of tissue-specific gene transcription in cancer cells promotes metastasis. Using colorectal cancer (CRC) as a model, we found in both clinical and cell line studies that metastatic CRC cells lose their colon-specific gene transcription program and gain a liver-specific gene transcription program as they metastasize in the liver. Further, we found this transcription reprogramming is driven by a reshaped epigenetic landscape of both typical and super-enhancers. Chemical inhibition of enhancer activity disrupts the ability of cells to execute altered transcription programs and consequently inhibits metastasis. Binding motif analysis of the enhancers in liver metastatic CRC cells identified the liver-specific transcription factor FOXA2 as a key regulator, and knocking down of FOXA2 expression prevents the colonization of metastatic CRC cells in the liver of a mice xenograft model. These results, together with additional observations of similar reprogramming in several cohorts of clinical CRC tumor samples and in multiple other forms of metastatic cancers, indicate that this reprogramming may be a common feature of metastasis in multiple cancers and suggest the targeted disruption of this epigenetic reprogramming as a strategy for the development of therapies to treat metastasis, the leading cause of cancer-related mortality.}, URL = {https://www.biorxiv.org/content/early/2017/04/26/131102}, eprint = {https://www.biorxiv.org/content/early/2017/04/26/131102.full.pdf}, journal = {bioRxiv} }