RT Journal Article
SR Electronic
T1 An efficient platform for astrocyte differentiation from human induced pluripotent stem cells
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 133496
DO 10.1101/133496
A1 TCW Julia
A1 Minghui Wang
A1 Anna A. Pimenova
A1 Kathryn R. Bowles
A1 Brigham J. Hartley
A1 Emre Lacin
A1 Saima Machlovi
A1 Rawan Abdelaal
A1 Celeste M. Karch
A1 Hemali Phetnani
A1 Paul A. Slesinger
A1 Bin Zhang
A1 Alison M. Goate
A1 Kristen J. Brennand
YR 2017
UL http://biorxiv.org/content/early/2017/05/03/133496.abstract
AB Growing evidence implicates the importance of glia, particularly astrocytes, in neurological and psychiatric diseases. Here, we describe a rapid and robust method for the differentiation of highly pure populations of replicative astrocytes from human induced pluripotent stem cells (hiPSCs), via a neural progenitor cell (NPC) intermediate. Using this method, we generated hiPSC-derived astrocyte populations (hiPSC-astrocytes) from 42 NPC lines (derived from 30 individuals) with an average of ∼90% S100β-positive cells. Transcriptomic analysis demonstrated that the hiPSC-astrocytes are highly similar to primary human fetal astrocytes and characteristic of a non-reactive state. hiPSC-astrocytes respond to inflammatory stimulants, display phagocytic capacity and enhance microglial phagocytosis. hiPSC-astrocytes also possess spontaneous calcium transient activity. Our novel protocol is a reproducible, straightforward (single media) and rapid (<30 days) method to generate homogenous populations of hiPSC-astrocytes that can be used for neuron-astrocyte and microglia-astrocyte co-cultures for the study of neuropsychiatric disorders.hiPSChuman induced pluripotent stem cellNPCneural progenitor cell