RT Journal Article
SR Electronic
T1 A high resolution whole brain imaging using Oblique Light Sheet Tomography
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 132423
DO 10.1101/132423
A1 Arun Narasimhan
A1 Kannan Umadevi Venkatraju
A1 Judith Mizrachi
A1 Dinu F. Albeanu
A1 Pavel Osten
YR 2017
UL http://biorxiv.org/content/early/2017/05/05/132423.abstract
AB We have developed a oblique version of the light sheet microscope to do volumetric imaging of the whole brains at high spatial resolution. Tissue clearing using aqueous techniques reduces the structural rigidity of the brain, to overcome this, we developed a gelatin based re-embedding procedure that makes the brain rigid enough to be sectioned. A few examples of whole brain volumetric images are shown using the oblique light sheet tomography.