RT Journal Article SR Electronic T1 3D single-molecule super-resolution microscopy with a tilted light sheet JF bioRxiv FD Cold Spring Harbor Laboratory SP 135699 DO 10.1101/135699 A1 Anna-Karin Gustavsson A1 Petar N. Petrov A1 Maurice Y. Lee A1 Yoav Shechtman A1 W. E. Moerner YR 2017 UL http://biorxiv.org/content/early/2017/05/09/135699.abstract AB Tilted light sheet microscopy with 3D point spread functions (TILT3D) combines a tilted light sheet illumination scheme and engineered point spread functions with axial ranges up to ten μm for low background, 3D super-localization of single molecules. This system is built upon a standard inverted microscope and has minimal custom parts. The result is simple and flexible 3D super-resolution imaging with tens of nm localization precision throughout thick mammalian cells.