RT Journal Article SR Electronic T1 High-density superresolution microscopy with an incoherent light source and a conventional epifluorescence microscope setup JF bioRxiv FD Cold Spring Harbor Laboratory SP 121061 DO 10.1101/121061 A1 Kirti Prakash YR 2017 UL http://biorxiv.org/content/early/2017/05/17/121061.abstract AB We report that single-molecule superresolution microscopy can be achieved with a conventional epifluorescence microscope setup and a Mercury arc lamp. The configuration termed as Omnipresent Localisation Microscope (OLM), is an extension of Single Molecule Localisation Microscopy (SMLM) techniques and allows single molecules to be switched on and off (a phenomenon termed as “blinking”), detected and localised. The use of a short burst of deep blue excitation (350-380 nm) can be further used to reactivate the blinking, once the blinking process has slowed or stopped. A resolution of 90 nm is achieved on test specimens (mouse and amphibian meiotic chromosomes). Finally, we demonstrate that STED and OLM can be performed on the same biological sample using a simple imaging medium. It is hoped that this type of correlative imaging will provide a basis for a further enhanced resolution.