RT Journal Article
SR Electronic
T1 Isolation and characterization of key genes that promote flavonoid accumulation in purple-leaf tea (<em>Camellia sinensis</em> L.)
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 139105
DO 10.1101/139105
A1 Xiujuan He
A1 Xuecheng Zhao
A1 Liping Gao
A1 Xingxing Shi
A1 Xinlong Dai
A1 Yajun Liu
A1 Tao Xia
A1 Yunsheng Wang
YR 2017
UL http://biorxiv.org/content/early/2017/05/17/139105.abstract
AB There were several high concentrations of flavonoid components in tea leaves that present health benefits. A novel purple-leaf tea variety, ‘Mooma1’, was obtained from the natural hybrid population of Longjing 43 variety. The buds and young leaves of ‘Mooma1’ were displayed in bright red. HPLC and LC-MS analysis showed that anthocyanins and O-Glycosylated flavonols were remarkably accumulated in the leaves of ‘Mooma1’, while the total amount of catechins in purple-leaf leaves was slightly decreased compared with the control. A R2R3-MYB transcription factor (CsMYB6A) and a novel UGT gene (CsUGT72AM1), that were highly expressed in purple leaf were isolated and identified by transcriptome sequencing. The over-expression of transgenic tobacco confirmed that CsMYB6A can activate the expression of flavonoid-related structural genes, especially CHS and 3GT, controlling the accumulation of anthocyanins in the leaf of transgenic tobacco. Enzymatic assays in vitro confirmed that CsUGT72AM1 has catalytic activity as a flavonol 3-O-glucosyltransferase, and displayed broad substrate specificity. The results were useful for further elucidating the molecular mechanisms of the flavonoid metabolic fluxes in the tea plant.