@article {McGorty140418, author = {Ryan McGorty and Dan Xie and Bo Huang}, title = {Aberration-corrected high-NA open-top selective-plane illumination microscopy for biological imaging}, elocation-id = {140418}, year = {2017}, doi = {10.1101/140418}, publisher = {Cold Spring Harbor Laboratory}, abstract = {Selective-plane illumination microscopy (SPIM) provides unparalleled advantages for volumetric imaging of living organisms over extended times. However, the spatial configuration of a SPIM system often limits its compatibility with many widely used biological sample holders such as multi-well chambers and plates. To solve this problem, we developed a high numerical aperture (NA) open-top configuration that places both the excitation and detection objectives on the opposite of the sample coverglass. We carried out a theoretical calculation to analyze the structure of the system-induced aberrations. We then experimentally showed aberration-correction using adaptive optics combined with static optical components, demonstrating neardiffraction-limited performance in imaging fluorescently labeled cells.}, URL = {https://www.biorxiv.org/content/early/2017/05/20/140418}, eprint = {https://www.biorxiv.org/content/early/2017/05/20/140418.full.pdf}, journal = {bioRxiv} }