RT Journal Article
SR Electronic
T1 A complete electron microscopy volume of the brain of adult Drosophila melanogaster
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 140905
DO 10.1101/140905
A1 Zhihao Zheng
A1 J. Scott Lauritzen
A1 Eric Perlman
A1 Camenzind G. Robinson
A1 Matthew Nichols
A1 Daniel Milkie
A1 Omar Torrens
A1 John Price
A1 Corey B. Fisher
A1 Nadiya Sharifi
A1 Steven A. Calle-Schuler
A1 Lucia Kmecova
A1 Iqbal J. Ali
A1 Bill Karsh
A1 Eric T. Trautman
A1 John Bogovic
A1 Philipp Hanslovsky
A1 Gregory S. X. E. Jefferis
A1 Michael Kazhdan
A1 Khaled Khairy
A1 Stephan Saalfeld
A1 Richard D. Fetter
A1 Davi D. Bock
YR 2017
UL http://biorxiv.org/content/early/2017/05/22/140905.abstract
AB Drosophila melanogaster has a rich repertoire of innate and learned behaviors. Its 100,000–neuron brain is a large but tractable target for comprehensive neural circuit mapping. Only electron microscopy (EM) enables complete, unbiased mapping of synaptic connectivity; however, the fly brain is too large for conventional EM. We developed a custom high-throughput EM platform and imaged the entire brain of an adult female fly. We validated the dataset by tracing brain-spanning circuitry involving the mushroom body (MB), intensively studied for its role in learning. Here we describe the complete set of olfactory inputs to the MB; find a new cell type providing driving input to Kenyon cells (the intrinsic MB neurons); identify neurons postsynaptic to Kenyon cell dendrites; and find that axonal arbors providing input to the MB calyx are more tightly clustered than previously indicated by light-level data. This freely available EM dataset will significantly accelerate Drosophila neuroscience.HIGHLIGHTS- A complete adult fruit fly brain was imaged, using electron microscopy (EM)- The EM volume enables brain-spanning mapping of neuronal circuits at the synaptic level- Olfactory projection neurons cluster more tightly in mushroom body calyx than expected from light-level data- The primary postsynaptic targets of Kenyon cells (KCs) in the MB are other KCs, as well as the anterior paired lateral (APL) neuron- A newly discovered cell type, MB-CP2, integrates input from several sensory modalities and provides microglomerular input to KCs in MB calyx- A software pipeline was created in which EM-traced skeletons can be searched for within existing large-scale light microscopy (LM) databases of neuronal morphology, facilitating cell type identification and discovery of relevant genetic driver lines