RT Journal Article
SR Electronic
T1 Distinct pattern of epigenetic DNA modification in leukocytes from patients with colorectal carcinoma and individuals with precancerous conditions, benign adenoma and inflammatory bowel disease – a link to oxidative stress
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 141903
DO 10.1101/141903
A1 Marta Starczak
A1 Ewelina Zarakowska
A1 Martyna Modrzejewska
A1 Tomasz Dziaman
A1 Anna Szpila
A1 Kinga Linowiecka
A1 Jolanta Guz
A1 Justyna Szpotan
A1 Maciej Gawronski
A1 Anna Labejszo
A1 Zbigniew Banaszkiewicz
A1 Maria Klopocka
A1 Marek Foksinski
A1 Daniel Gackowski
A1 Ryszard Olinski
YR 2017
UL http://biorxiv.org/content/early/2017/05/25/141903.abstract
AB A characteristic feature of malignant cells, including colorectal cancer cells, is a profound decrease in level of 5-hydroxymethylcytosine, product of 5-methylcytosine oxidation by TET enzymes. This study included four groups of subjects: healthy controls, and patients with inflammatory bowel disease (IBD), benign polyps and colorectal cancer. Patients from all groups presented with significantly lower levels of 5-methylcytosine and 5-hydroxymethylcytosine than the controls. A similar tendency was also observed for 5-hydroxymethyluracil level. Patients with IBD showed the highest levels of 5-formylcytosine and 8-oxo-7,8-dihydro-2’-deoxyguanosine of all study subjects, and individuals with colorectal cancer presented with the lowest concentrations of vitamin C and A. Expressions of TET1 and TET2 turned out to be the highest in IBD group. To the best of our knowledge, this is the first study to show that healthy subjects, individuals with precancerous conditions and colorectal cancer patients present with distinct specific patterns of epigenetic modifications in leukocyte DNA.