TY - JOUR T1 - Wireless implantable sensor for non-invasive, longitudinal quantification of axial strain across rodent long bone defects JF - bioRxiv DO - 10.1101/142778 SP - 142778 AU - Brett S. Klosterhoff AU - Keat Ghee Ong AU - Laxminarayanan Krishnan AU - Kevin M. Hetzendorfer AU - Young-Hui Chang AU - Mark G. Allen AU - Robert E. Guldberg AU - Nick J. Willett Y1 - 2017/01/01 UR - http://biorxiv.org/content/early/2017/05/26/142778.abstract N2 - Bone development, maintenance, and regeneration are remarkably sensitive to mechanical cues. Consequently, mechanical stimulation has long been sought as a putative target to promote endogenous healing after fracture. Given the transient nature of bone repair, tissue-level mechanical cues evolve rapidly over time after injury and are challenging to measure non-invasively. The objective of this work was to develop and characterize an implantable strain sensor for non-invasive monitoring of axial strain across a rodent femoral defect during functional activity. Herein, we present the design, characterization, and in vivo demonstration of the device’s capabilities for quantitatively interrogating physiological dynamic strains during bone regeneration. Ex vivo experimental characterization of the device showed that it exceeded the technical requirements for sensitivity, signal resolution, and electromechanical stability. The digital telemetry minimized power consumption, enabling long-term intermittent data collection. Devices were implanted in a rat 6 mm femoral segmental defect model and after three days, data were acquired wirelessly during ambulation and synchronized to corresponding radiographic videos, validating the ability of the sensor to non-invasively measure strain in real-time. Lastly, in vivo strain measurements were utilized in a finite element model to estimate the strain distribution within the defect region. Together, these data indicate the sensor is a promising technology to quantify local tissue mechanics in a specimen specific manner, facilitating more detailed investigations into the role of the mechanical environment in dynamic skeletal healing and remodeling. ER -