RT Journal Article
SR Electronic
T1 Spatial specificity of auxin responses coordinates wood formation
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 142885
DO 10.1101/142885
A1 Klaus Brackmann
A1 Virginie Jouannet
A1 Jiyan Qi
A1 Theresa Schlamp
A1 Karin Grünwald
A1 Pablo Sanchez
A1 Thomas Greb
YR 2017
UL http://biorxiv.org/content/early/2017/05/27/142885.abstract
AB Spatial organization of signaling events of the phytohormone auxin is fundamental for maintaining a dynamic transition from plant stem cells to differentiated descendants. The cambium, the stem cell niche mediating wood formation, fundamentally depends on auxin signaling but its exact role and spatial organization is obscure. Here, we show that, while auxin signaling levels increase in differentiating cambium descendants, a moderate level of signaling in cambial stem cells is essential for cambium activity. We identify the auxin-dependent transcription factor ARF5/MONOPTEROS to cell-autonomously restrict the number of stem cells by attenuating the activity of the stem cell promoting WOX4 gene. In contrast, ARF3 and ARF4 function as cambium activators in a redundant fashion from outside of WOX4-expressing cells. Our results reveal an influence of auxin signaling on distinct cambium features by specific signaling components and allow the conceptual integration of plant stem cell systems with distinct anatomies.