TY - JOUR T1 - Lipid transfer from plants to arbuscular mycorrhiza fungi JF - bioRxiv DO - 10.1101/143883 SP - 143883 AU - Andreas Keymer AU - Priya Pimprikar AU - Vera Wewer AU - Claudia Huber AU - Mathias Brands AU - Simone L. Bucerius AU - Pierre-Marc Delaux AU - Verena Klingl AU - Edda von Röpenack-Lahaye AU - Trevor L. Wang AU - Wolfgang Eisenreich AU - Peter Dörmann AU - Martin Parniske AU - Caroline Gutjahr Y1 - 2017/01/01 UR - http://biorxiv.org/content/early/2017/05/30/143883.abstract N2 - Arbuscular mycorrhiza (AM) symbioses contribute to global carbon cycles as plant hosts divert up to 20% of photosynthate to the obligate biotrophic fungi. Previous studies suggested carbohydrates as the only form of carbon transferred to the fungi. However, de novo fatty acid (FA) synthesis has not been observed in AM fungi in absence of the plant. In a forward genetic approach, we identified two Lotus japonicus mutants defective in AM-specific paralogs of lipid biosynthesis genes (KASI and GPAT6). These mutants perturb fungal development and accumulation of emblematic fungal 16:1ω5 FAs. Using isotopolog profiling we demonstrate that 13C patterns of fungal FAs recapitulate those of wild-type hosts, indicating cross-kingdom lipid transfer from plants to fungi. This transfer of labelled FAs was not observed for the AM-specific lipid biosynthesis mutants. Thus, growth and development of beneficial AM fungi is not only fueled by sugars but depends on lipid transfer from plant hosts. ER -