PT  - JOURNAL ARTICLE
AU  - Eun Ji Kim
AU  - Gregory G. Grant
AU  - Anita S. Bowman
AU  - Naqi Haider
AU  - Harini V. Gudiseva
AU  - Venkata Ramana Murthy Chavali
TI  - Complete Transcriptome Profiling of Normal and Age-Related Macular Degeneration Eye Tissues Reveals Changes in Regulation of Non-Coding RNA and Extreme Disregulation of Anti-Sense Transcription
AID  - 10.1101/143917
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 143917
4099  - http://biorxiv.org/content/early/2017/05/30/143917.short
4100  - http://biorxiv.org/content/early/2017/05/30/143917.full
AB  - Strand specific RNA sequencing of retina and RPE-Choroid-Scleara (RCS) in age-related macular degeneration (AMD) and matched normal controls reveals striking impact on anti-sense transcription and changes in the regulation of non-coding RNA that has not previously been reported. Hundreds of genes, which do not express anti-sense transcripts in normal retina and RCS, demonstrate extreme anti-sense expression in AMD. And conversely anti-sense transcription is completely abrogated in many genes which express a high level of anti-sense transcripts in normal retina and RCS. Several pathways are very highly enriched in the upregulated anti-sense transcripts - in particular the EIF2 signaling pathway. These results call for a deeper investigation into anti-sense and noncoding RNA regulation in AMD and their potential as therapeutic targets.