RT Journal Article SR Electronic T1 Complete Transcriptome Profiling of Normal and Age-Related Macular Degeneration Eye Tissues Reveals Changes in Regulation of Non-Coding RNA and Extreme Disregulation of Anti-Sense Transcription JF bioRxiv FD Cold Spring Harbor Laboratory SP 143917 DO 10.1101/143917 A1 Eun Ji Kim A1 Gregory G. Grant A1 Anita S. Bowman A1 Naqi Haider A1 Harini V. Gudiseva A1 Venkata Ramana Murthy Chavali YR 2017 UL http://biorxiv.org/content/early/2017/05/30/143917.abstract AB Strand specific RNA sequencing of retina and RPE-Choroid-Scleara (RCS) in age-related macular degeneration (AMD) and matched normal controls reveals striking impact on anti-sense transcription and changes in the regulation of non-coding RNA that has not previously been reported. Hundreds of genes, which do not express anti-sense transcripts in normal retina and RCS, demonstrate extreme anti-sense expression in AMD. And conversely anti-sense transcription is completely abrogated in many genes which express a high level of anti-sense transcripts in normal retina and RCS. Several pathways are very highly enriched in the upregulated anti-sense transcripts - in particular the EIF2 signaling pathway. These results call for a deeper investigation into anti-sense and noncoding RNA regulation in AMD and their potential as therapeutic targets.