TY - JOUR T1 - The Human Cytoplasmic Dynein Interactome Reveals Novel Activators of Motility JF - bioRxiv DO - 10.1101/143743 SP - 143743 AU - William B. Redwine AU - Morgan E. DeSantis AU - Ian Hollyer AU - Zaw Min Htet AU - Phuoc Tien Tran AU - Selene K. Swanson AU - Laurence Florens AU - Michael P. Washburn AU - Samara L. Reck-Peterson Y1 - 2017/01/01 UR - http://biorxiv.org/content/early/2017/05/30/143743.abstract N2 - In human cells, cytoplasmic dynein-1 is essential for long-distance transport of many cargos, including organelles, RNAs, proteins, and viruses, towards microtubule minus ends. To understand how a single motor achieves cargo specificity, we identified the human dynein interactome or “transportome” by attaching a promiscuous biotin ligase (“BioID”) to seven components of the dynein machinery, including a subunit of the essential cofactor dynactin. This method reported spatial information about the large cytosolic dynein/dynactin complex in living cells. To achieve maximal motile activity and to bind its cargos, human dynein/dynactin requires “activators”, of which only five have been described. We developed methods to identify new activators in our BioID data, and discovered that ninein and ninein-like are a new family of dynein activators. Analysis of the protein interactomes for six activators, including ninein and ninein-like, suggests that each dynein activator has multiple cargos. ER -