PT  - JOURNAL ARTICLE
AU  - Xiaohong Jing
AU  - Weiqing Wang
AU  - Nicholas P. Gauthier
AU  - Poorvi Kaushik
AU  - Alex Root
AU  - Richard R. Stein
AU  - Anil Korkut
AU  - Chris Sander
TI  - Protein profiling in cancer cell lines and tumor tissue using reverse phase protein arrays
AID  - 10.1101/144535
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 144535
4099  - http://biorxiv.org/content/early/2017/05/31/144535.short
4100  - http://biorxiv.org/content/early/2017/05/31/144535.full
AB  - Reverse phase protein array (RPPA) technology is an antibody-based high-throughput assay for protein profiling of biological specimens that allows for many measurements with very small amounts of cell lysate. Here, we report the sensitivity, reproducibility, and accuracy of a particular RPPA platform called Zeptosens. We customized the RPPA protocol for our in-house setup, and measured more than 80 total protein and phospho-protein levels in various cancer samples, including cell lines, organoids, tumor chunks, core needle biopsies, and laser-capture microdissected tissue samples. We discuss pros and cons of the RPPA platform, and describe results from profiling 15 cancer cell line cells using RPPA.