RT Journal Article
SR Electronic
T1 Cyclin G and the Polycomb Repressive Complexes PRC1 and PR-DUB cooperate for developmental stability
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 144048
DO 10.1101/144048
A1 Delphine Dardalhon-Cuménal
A1 Jérôme Deraze
A1 Camille A Dupont
A1 Valérie Ribeiro
A1 Anne Coléno-Costes
A1 Juliette Pouch
A1 Stéphane Le Crom
A1 Hélène Thomassin
A1 Vincent Debat
A1 Neel B Randsholt
A1 Frédérique Peronnet
YR 2017
UL http://biorxiv.org/content/early/2017/06/01/144048.abstract
AB In Drosophila, ubiquitous expression of a short Cyclin G isoform generates extreme developmental noise estimated by fluctuating asymmetry (FA), providing a model to tackle developmental stability. This transcriptional cyclin interacts with chromatin regulators of the Enhancer of Trithorax and Polycomb (ETP) and Polycomb families. This led us to investigate the importance of these interactions in developmental stability. Deregulation of Cyclin G highlights an organ intrinsic control of developmental noise, linked to the ETP-interacting domain, and enhanced by mutations in genes encoding members of the Polycomb Repressive complexes PRC1 and PR-DUB. Deep-sequencing of wing imaginal discs deregulating CycG reveals that high developmental noise correlates with up-regulation of genes involved in translation and down-regulation of genes involved in energy production. Most Cyclin G direct transcriptional targets are also direct targets of PRC1 and RNAPolII in the developing wing. Altogether, our results suggest that Cyclin G, PRC1 and PR-DUB cooperate for developmental stability.