PT  - JOURNAL ARTICLE
AU  - Nicole Toepfner
AU  - Christoph Herold
AU  - Oliver Otto
AU  - Philipp Rosendahl
AU  - Angela Jacobi
AU  - Martin Kräter
AU  - Julia Stächele
AU  - Leonhard Menschner
AU  - Maik Herbig
AU  - Laura Ciuffreda
AU  - Lisa Ranford-Cartwright
AU  - Michal Grzybek
AU  - Ünal Coskun
AU  - Elisabeth Reithuber
AU  - Geneviève Garriss
AU  - Peter Mellroth
AU  - Birgitta Henriques-Normark
AU  - Nicola Tregay
AU  - Meinolf Suttorp
AU  - Martin Bornhäuser
AU  - Edwin R. Chilvers
AU  - Reinhard Berner
AU  - Jochen Guck
TI  - Detection of human disease conditions by single-cell morpho-rheological phenotyping of whole blood
AID  - 10.1101/145078
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 145078
4099  - http://biorxiv.org/content/early/2017/06/01/145078.short
4100  - http://biorxiv.org/content/early/2017/06/01/145078.full
AB  - Blood is arguably the most important bodily fluid and its analysis provides crucial health status information. A first routine measure to narrow down diagnosis in clinical practice is the differential blood count, determining the frequency of all major blood cells. What is lacking to advance initial blood diagnostics is an unbiased and quick functional assessment of blood that can narrow down the diagnosis and generate specific hypotheses. To address this need, we introduce the continuous, cell-by-cell morpho-rheological (MORE) analysis of whole blood, without labeling, enrichment or separation, at rates of 1,000 cells/sec. In a drop of blood we can identify all major blood cells and characterize their pathological changes in several disease conditions in vitro and in patient samples. This approach takes previous results of mechanical studies on specifically isolated blood cells to the level of application directly in whole blood and adds a functional dimension to conventional blood analysis.