PT  - JOURNAL ARTICLE
AU  - Yusuke Tomina
AU  - Daniel A. Wagenaar
TI  - Whole-ganglion imaging of voltage in the medicinal leech using a double-sided microscope
AID  - 10.1101/145144
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 145144
4099  - http://biorxiv.org/content/early/2017/06/02/145144.short
4100  - http://biorxiv.org/content/early/2017/06/02/145144.full
AB  - Studies of neuronal network emergence during sensory processing and motor control are greatly promoted by technologies that allow us to simultaneously record the membrane potential dynamics of a large population of neurons in single cell resolution. To achieve whole-brain recording with the ability to detect both small synaptic potentials and action potentials, we developed a voltage-sensitive dye (VSD) imaging technique based on a double-sided microscope that can image two sides of a nervous system simultaneously. We applied this system to the segmental ganglia of the medicinal leech. Double-sided VSD imaging enabled simultaneous recording of membrane potential events from almost all of the identifiable neurons. Using data obtained from double-sided VSD imaging we analyzed neuronal dynamics in both sensory processing and generation of behavior and constructed functional maps for identification of neurons contributing to these processes.