TY - JOUR T1 - BEARscc determines robustness of single-cell clusters using simulated technical replicates JF - bioRxiv DO - 10.1101/118919 SP - 118919 AU - DT Severson AU - RP Owen AU - MJ White AU - X Lu AU - B Schuster-Böckler Y1 - 2017/01/01 UR - http://biorxiv.org/content/early/2017/06/05/118919.abstract N2 - Technical variance is a major confounding factor in single-cell RNA sequencing, not least because it is not possible to replicate measurements on the same cell. We present BEARscc, a tool that uses RNA spike-in controls to simulate experiment-specific technical replicates. We demonstrate that the tool improves the unsupervised classification of cells and facilitates the biological interpretation of single-cell RNA-seq experiments. ER -