TY - JOUR T1 - Nitric oxide sensing by chlorophyll <em>a</em> JF - bioRxiv DO - 10.1101/146563 SP - 146563 AU - Abhishek Bhattacharya AU - Pranjal Biswas AU - Puranjoy Kar AU - Piya Roychoudhury AU - Sankar Basu AU - Sanjay Ghosh AU - Kaustab Panda AU - Ruma Pal AU - Anjan Kr. Dasgupta Y1 - 2017/01/01 UR - http://biorxiv.org/content/early/2017/06/06/146563.abstract N2 - Nitric oxide (NO) acts as a signalling molecule that has direct and indirect regulatory roles in various functional processes in biology, though in plant kingdom its role is relatively unexplored. One reason for this is the fact that sensing of NO is always challenging. There are very few probes that can classify the different NO species. The present paper proposes a simple but straightforward way for sensing different NO species using chlorophyll, the source of inspiration being hemoglobin that serves as a NO sink in most mamalian system. The proposed method is able to classify NO from DETA-NONOate or (Z)-1-[N-(2-aminoethyl)-N-(2-ammonioethyl) amino] diazen-1-ium-1,2-diolate, nitrite, nitrate and S-nitrosothiol or SNO. This discrimination is carried out by chlorophyll a (chl a) at nano molar (nM) order of sensitivity and at 293K to 310K. Molecular docking reveals the differential binding behaviour of NO and SNO with chlorophyll, the predicted binding affinity matching with the experimental observation. Additional expreiments with diverse range of cyanobacteria reveals that apart from spectroscopic approach the proposed sensing module can be used in microscopic inspection of NO speices. Binding of NO is sensitive to tempertaure and static magnetic field. This provides additional support to the involvement of the porphyrin ring structure to the NO sensing process. This also broadens the scope of the sensing methods as hinted in the text. ER -