PT  - JOURNAL ARTICLE
AU  - Marta Starczak
AU  - Ewelina Zarakowska
AU  - Martyna Modrzejewska
AU  - Tomasz Dziaman
AU  - Anna Szpila
AU  - Kinga Linowiecka
AU  - Jolanta Guz
AU  - Justyna Szpotan
AU  - Maciej Gawronski
AU  - Anna Labejszo
AU  - Ariel Liebert
AU  - Zbigniew Banaszkiewicz
AU  - Maria Klopocka
AU  - Marek Foksinski
AU  - Daniel Gackowski
AU  - Ryszard Olinski
TI  - Distinct pattern of epigenetic DNA modification in leukocytes from patients with colorectal carcinoma and individuals with precancerous conditions, benign adenoma and inflammatory bowel disease – a link to oxidative stress
AID  - 10.1101/141903
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 141903
4099  - http://biorxiv.org/content/early/2017/06/11/141903.short
4100  - http://biorxiv.org/content/early/2017/06/11/141903.full
AB  - A characteristic feature of malignant cells, including colorectal cancer cells, is a profound decrease in level of 5-hydroxymethylcytosine, product of 5-methylcytosine oxidation by TET enzymes. This study included four groups of subjects: healthy controls, and patients with inflammatory bowel disease (IBD), benign polyps and colorectal cancer. Patients from all groups presented with significantly lower levels of 5-methylcytosine and 5-hydroxymethylcytosine than the controls. A similar tendency was also observed for 5-hydroxymethyluracil level. Patients with IBD showed the highest levels of 5-formylcytosine and 8-oxo-7,8-dihydro-2’-deoxyguanosine of all study subjects, and individuals with colorectal cancer presented with the lowest concentrations of vitamin C and A. Expressions of TET1 and TET2 turned out to be the highest in IBD group. To the best of our knowledge, this is the first study to show that healthy subjects, individuals with precancerous conditions and colorectal cancerpatients present with distinct specific patterns of epigenetic modifications in leukocyte DNA.