RT Journal Article
SR Electronic
T1 Identification of a native novel oncolytic immunoglobulin on exfoliated colon epithelial cells: A bispecific heterodimeric chimera of IgA/IgG
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 140673
DO 10.1101/140673
A1 George P. Albaugh
A1 Sudhir K. Dutta
A1 Vasantha Iyengar
A1 Samina Shami
A1 Althaf Lohani
A1 Eduardo Sainz
A1 George Kessie
A1 Prasanna Nair
A1 Sara Lagerholm
A1 Alka Kamra
A1 J-H Joshua Chen
A1 Shilpa Kalavapudi
A1 Robert Shores
A1 Laila E. Phillips
A1 Ram Nair
A1 Padmanabhan P Nair
YR 2017
UL http://biorxiv.org/content/early/2017/06/12/140673.abstract
AB Understanding the nature of cell surface markers on exfoliated colonic cells is a crucial step in establishing criteria for a normally functioning mucosa. We have found that colonic cells isolated from stool samples (SCSR-010 Fecal Cell Isolation Kit, NonInvasive Technologies, Elkridge, MD), preserved at room temperature for up to one week, with viability of >85% and low levels of apoptosis (8% - 10%) exhibit two distinct cell size subpopulations, in the 2.5μM– 5.0 μM and 5.0μM-8.0μM range. In addition to IgA, about 60% of the cells expressed a novel heterodimeric IgA/IgG immunoglobulin that conferred a broad-spectrum cell mediated cytotoxicity against tumor cells. In a cohort of 58 subjects the exclusive absence of this immunoglobulin in two African-Americans was suggestive of a germline deletion. Serial cultures in stem cell medium retained the expression of this heterodimer. Since a majority of the cystic cells expressed the stem cell markers Lgr5 and Musashi-1 we termed these cells as gastrointestinal progenitor stem cells (GIP-C**). CXCR-4, the cytokine co-receptor for HIV was markedly expressed. These cells also expressed CD20, IgA, IgG, CD45, and COX-2. We assume that they originated from mature columnar epithelium by dedifferentiation. Our observations indicate that we have a robust noninvasive method to study mucosal pathophysiology and a direct method to create a database for applications in regenerative medicine.