RT Journal Article
SR Electronic
T1 Low-cost HPV testing and the prevalence of cervical infection in asymptomatic populations in Guatemala
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 134775
DO 10.1101/134775
A1 Hong Lou
A1 Eduardo Gharzouzi
A1 Sarita Polo Guerra
A1 Joël Fokom Domgue
A1 Julie Sawitzke
A1 Guillermo Villagran
A1 Lisa Garland
A1 Joseph Boland
A1 Sarah Wagner
A1 Joanna Martinez
A1 Héctor Rosas
A1 Jami Troxler
A1 Heidi McMillen
A1 Bailey Kessing
A1 Enrique Alvirez
A1 Miriam Castillo
A1 Hesler Morales
A1 Victor Argueta
A1 Andert Rosingh
A1 Femke J.H.B. van Aerde-van Nunen
A1 Griselda Lopez
A1 Mark Schiffman
A1 Michael Dean
A1 Roberto Orozco
YR 2017
UL http://biorxiv.org/content/early/2017/06/13/134775.abstract
AB Background A low cost and accurate method for detecting high-risk (HR) HPV is important to permit HPV testing for cervical cancer prevention. We validated a low-cost commercially available HPV method (H13, Hybribio, Hong Kong) and determined the distribution of HPV infections in over 1717 cancer-free women in Guatemala.Methods H13 results were compared with two more established HPV tests: (Xpert™ (Cepheid) and SPF10-LIPA25™ (DDL)) in 40 mainly known positive specimens. HR-HPV was detected in cervical samples from 1717 cancer-free women receiving Pap smears using the Hybribio™ real-time PCR assay of 13 HR types. Selected HPV positive samples were sequenced to determine viral type.Results The Hybribio H13 Assay showed 93% identical results with Xpert, and 89% with SPF1 0-LIPA25. A total of 13% (226/1717) of women tested HPV+. The highest prevalence was found in younger women (<30 years, 22 %) and older ones (≥ 60 years, 15%). The six most common HR-HPV types among the 148 HPV+ typed were HPV16 (22%), HPV18 (11%), HPV39 (11%), HPV58 (10%), HPV52 (8%), and HPV45 (8%).Conclusions The Hybribio Assay was low cost, and accurate in testing for HR-HPV infection. As in most places, HPV16 was the most prevalent HR type in Guatemala and the age-specific prevalence curve peaked in younger ages.A low cost and accurate method, Hybribio Assay, could be used for CC testing in low income regions.A total of 13% of cancer-free women were HPV+ and positivity was associated with younger age (<30 years old) in Guatemala.HPV16 was the major prevalent type.