RT Journal Article
SR Electronic
T1 Arabidopsis U1 snRNP subunit LUC7 functions in alternative splicing and preferential removal of terminal introns
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 150805
DO 10.1101/150805
A1 Marcella de Francisco Amorim
A1 Eva-Maria Willing
A1 Emese Xochitl Szabo
A1 Irina Droste-Borel
A1 Boris Maček
A1 Korbinian Schneeberger
A1 Sascha Laubinger
YR 2017
UL http://biorxiv.org/content/early/2017/06/16/150805.abstract
AB Introns are removed by the spliceosome, a large complex composed of five ribonucleoprotein subcomplexes (U snRNP). The U1 snRNP, which binds to 5’ splice sites, plays an essential role in early steps of the splicing reaction. Here, we show that Arabidopsis LUC7 is a U1 snRNP subunit that affects constitutive and alternative splicing. Interestingly, LUC7 specifically promotes splicing of a subset of terminal introns. Splicing of LUC7-dependent terminal introns is a prerequisite for nuclear export and can be modulated by stress. Globally, intron retention under stress conditions occurs preferentially among first and terminal introns, uncovering an unknown bias for splicing regulation in Arabidopsis. Taken together, our study reveals that the Arabidopsis U1 snRNP component LUC7 is important for alternative splicing and removal of terminal introns and it suggests that Arabidopsis terminal introns fine-tune gene expression under stress conditions.