RT Journal Article
SR Electronic
T1 Whole-ganglion imaging of voltage in the medicinal leech using a double-sided microscope
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 145144
DO 10.1101/145144
A1 Yusuke Tomina
A1 Daniel A. Wagenaar
YR 2017
UL http://biorxiv.org/content/early/2017/06/19/145144.abstract
AB Studies of neuronal network emergence during sensory processing and motor control are greatly promoted by technologies that allow us to simultaneously record the membrane potential dynamics of a large population of neurons in single cell resolution. To achieve whole-brain recording with the ability to detect both small synaptic potentials and action potentials, we developed a voltage-sensitive dye (VSD) imaging technique based on a double-sided microscope that can image two sides of a nervous system simultaneously. We applied this system to the segmental ganglia of the medicinal leech. Double-sided VSD imaging enabled simultaneous recording of membrane potential events from almost all of the identifiable neurons. Using data obtained from double-sided VSD imaging we analyzed neuronal dynamics in both sensory processing and generation of behavior and constructed functional maps for identification of neurons contributing to these processes.