PT - JOURNAL ARTICLE AU - Lukasz G. Migas AU - Aidan P. France AU - Bruno Bellina AU - Perdita E. Barran TI - <em>ORIGAMI</em>: A Software Suite for Activated Ion Mobility Mass Spectrometry (aIM-MS) Applied To Multimeric Protein Assemblies AID - 10.1101/152686 DP - 2017 Jan 01 TA - bioRxiv PG - 152686 4099 - http://biorxiv.org/content/early/2017/06/20/152686.short 4100 - http://biorxiv.org/content/early/2017/06/20/152686.full AB - We present here a software suite (ORIGAMI) that facilitates the rapid acquisition and analysis of ion mobility data following collisional activation. ORIGAMI was developed for use on Waters Synapt instruments where data acquisition is achieved by interfacing WREnS (Waters Research Enabled Software) and MassLynx. Two components are presented, the first is ORIGAMIMS which enables activation of ions by sequential increase of collision voltages prior to ion mobility analysis. We demonstrate the use of ORIGAMI on the tetrameric assemblies formed by the proteins concanavalin A (103 kDa) and alcohol dehydrogenase (143 kDa). Activation is performed in the trap collision cell of the Synapt TriWave assembly, where the collision voltage can be ramped from 0-200 V. All of the acquired data is recorded in a single file which simplifies data acquisition. This substantially decreases the time needed to perform a typical activated IM-MS experiment on a single protein charge state from approx. 2 hours to ~25 minutes. Following data acquisition the data is analysed in the second component, ORIGAMIANALYSE, which allows the user to visualise the effect of activation on the mobility of the parent ion, as well as on any produced fragment ion. The user can export the data in the form of heat maps, waterfall or wire plots. In addition, tools implemented in ORIGAMI enable easy data extraction from single or multiple MassLynx .raw files, in-depth interrogation of large datasets, statistical analysis and figure creation capabilities. We demonstrate the use of ORIGAMI on concanavalin A and alcohol dehydrogenase acquired using the traditional protocols and the ORIGAMIMS method.Highlights- New methodology for faster acquisition and data analysis following activation of ions separated by ion mobility mass spectrometry- Software package capable of simultaneous analysis of multiple MassLynx .raw files- Visualization of the change in mobility of parent and fragment ions following activation- Easy extraction, data processing and extensive plotting tools