RT Journal Article
SR Electronic
T1 Emergent properties in complex synthetic bacterial promoters
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 152934
DO 10.1101/152934
A1 Lummy Maria Oliveira Monteiro
A1 Letícia Magalhães Arruda
A1 Rafael Silva-Rocha
YR 2017
UL http://biorxiv.org/content/early/2017/06/20/152934.abstract
AB Regulation of gene expression in bacteria results from the interplay between transcriptional factors (TFs) at target promoters, and how the arrangement of binding sites determines the regulatory logic of promoters is not well known. Here, we generated and fully characterized a library of synthetic complex promoters for the global regulators, CRP and IHF, in Escherichia coli, formed by a weak -35/-10 consensus sequence preceded by four combinatorial binding sites for these TFs. We found that while cis-elements for CRP preferentially activate promoters when located immediately upstream of the promoter consensus, binding sites for IHF mainly function as “UP” elements and stimulate transcription in several different architectures in the absence of this protein. However, the combination of CRP- and IHF-binding sites resulted in emergent properties in these complex promoters, where the activity of combinatorial promoters cannot be predicted from the individual behavior of its components. Taken together, the results presented here add to the information on architecture-logic of complex promoters in bacteria.