PT  - JOURNAL ARTICLE
AU  - Linlin Zhang
AU  - Robert D. Reed
TI  - A practical guide to CRISPR/Cas9 genome editing in Lepidoptera
AID  - 10.1101/130344
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 130344
4099  - http://biorxiv.org/content/early/2017/06/22/130344.short
4100  - http://biorxiv.org/content/early/2017/06/22/130344.full
AB  - CRISPR/Cas9 genome editing has revolutionized functional genetic work in many organisms and is having an especially strong impact in emerging model systems. Here we summarize recent advances in applying CRISPR/Cas9 methods in Lepidoptera, with a focus on providing practical advice on the entire process of genome editing from experimental design through to genotyping. We also describe successful targeted GFP knockins that we have achieved in butterflies. Finally, we provide a complete, detailed protocol for producing targeted long deletions in butterflies.