PT  - JOURNAL ARTICLE
AU  - Jackson Z Lee
AU  - R Craig Everroad
AU  - Ulas Karaoz
AU  - Angela M Detweiler
AU  - Jennifer Pett-Ridge
AU  - Peter K Weber
AU  - Leslie Prufert-Bebout
AU  - Brad M Bebout
TI  - Metagenomics reveals niche partitioning within the phototrophic zone of a microbial mat
AID  - 10.1101/151704
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 151704
4099  - http://biorxiv.org/content/early/2017/06/26/151704.short
4100  - http://biorxiv.org/content/early/2017/06/26/151704.full
AB  - Hypersaline photosynthetic microbial mats are stratified microbial communities known for their taxonomic and metabolic diversity and strong light-driven day-night environmental gradients. In this study of the upper photosynthetic zone of hypersaline microbial mats of Elkhorn Slough, California (USA), we show how reference-based and reference-free methods can be used to meaningfully assess microbial ecology and genetic partitioning in these complex microbial systems. Mapping of metagenome reads to the dominant Cyanobacteria observed in the system, Coleofasciculus (Microcoleus) chthonoplastes, was used to examine strain variants within these metagenomes. Highly conserved gene subsystems indicate a core genome for the species, and a number of variant genes and subsystems suggest strain level differentiation, especially for carbohydrate utilization. Metagenome sequence coverage binning was used to assess ecosystem partitioning of remaining microbes. Functional gene annotation of these bins (primarily of Proteobacteria, Bacteroidetes, and Cyanobacteria) recapitulated the known biogeochemical functions in microbial mats using a genetic basis, and also revealed evidence of novel functional diversity within the Gemmatimonadetes and Gammaproteobacteria. Combined, these two approaches show how genetic partitioning can inform biogeochemical partitioning of the metabolic diversity within microbial ecosystems.